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HLA-B*27 (Human Leucocyte Antigen B27) DNA Detection by RT-PCR, EDTA Blood
₹ 3800
HLA-B*27 PCR
₹ 3800 ₹ 3800

HLA-B*27 PCR

Sample Type 51+ booked in last 3 days
Precaution
Sample Type
Swab
Precaution
Fasting
Yes
Precaution
Report
Within 4 Weeks

HLA-B*27 (Human Leucocyte Antigen B27) DNA Detection by RT-PCR, EDTA Blood Price

₹ 3800

Brief Details

Preparation
No specific preparation required
Also Known As
Human Leukocyte Antigen B27 Test, HLA-B27 DNA Test, HLA-B27 Gene Test
Fasting
Fasting not required
Reporting Time
Within 24 hours
Purpose
Detects the presence of the HLA-B27 gene associated with autoimmune conditions such as Ankylosing Spondylitis and reactive arthritis

What is an HLA B27 PCR Test?

The HLA B27 PCR Test is a genetic blood test that detects the presence of the Human Leukocyte Antigen B27 (HLA-B27) gene in your DNA. This gene is associated with an increased risk of developing certain autoimmune and inflammatory conditions, such as Ankylosing Spondylitis, Reactive Arthritis, and Juvenile Idiopathic Arthritis.

The test uses Polymerase Chain Reaction (PCR) technology to amplify and detect specific DNA sequences accurately, making it highly sensitive and reliable. Although carrying the HLA-B27 gene increases your risk, not everyone with the gene develops disease.

What Does an HLA B27 PCR Test Measure?

The test measures the presence or absence of the HLA-B27 gene in your white blood cells using PCR analysis.

It helps your doctor to:

  • Detect genetic susceptibility to autoimmune diseases.
  • Support diagnosis of conditions like Ankylosing Spondylitis or Reactive Arthritis.
  • Differentiate between inflammatory and non-inflammatory causes of joint pain.
  • Assess family members with similar symptoms or a history of autoimmune disorders.

HLA B27 PCR Test: Who Needs It and Why It’s Done

What Symptoms May Call for an HLA B27 PCR Test?

Your doctor may recommend this test if you experience symptoms suggestive of inflammatory joint disease such as:

  • Persistent lower back pain or stiffness, especially in the morning.
  • Swelling or pain in large joints like hips, knees, or shoulders.
  • Inflammation of the eyes (uveitis) causing redness or blurred vision.
  • Fatigue or general discomfort associated with autoimmune activity.

Who Should Get an HLA B27 PCR Test?

You may be advised to take this test if you:

  • Have a family history of autoimmune conditions.
  • Show early signs of spondyloarthropathies such as Ankylosing Spondylitis.
  • Are being evaluated for recurrent joint inflammation or chronic back pain.
  • Are diagnosed with uveitis or reactive arthritis for confirmation.
  • Are under investigation for autoimmune-related inflammatory disorders.

Why is an HLA B27 PCR Test Done?

The HLA B27 PCR Test helps determine whether you carry the HLA-B27 gene that can trigger abnormal immune responses. It assists doctors in diagnosing and monitoring autoimmune conditions and in differentiating genetic predisposition from acquired diseases.

Importance of an HLA B27 PCR Test

Identifying the HLA-B27 gene early can help guide your doctor in making an accurate diagnosis and initiating appropriate management strategies. While the gene itself does not cause disease, its presence indicates an increased risk for developing autoimmune disorders. Knowing your HLA-B27 status helps in monitoring symptoms, predicting disease patterns, and choosing targeted treatments to reduce inflammation and improve long-term joint health.

HLA B27 PCR Test Booking & Reports – Metropolis Healthcare

How to Book the HLA B27 PCR Test and Get Your Reports?

  1. Simple Online Booking
    Book the HLA B27 PCR Test through the Metropolis Healthcare website or mobile app. Select your preferred time slot and choose home collection or lab visit as per convenience.
  2. Safe Home Sample Collection
    Our trained phlebotomists collect your sample following all hygiene and safety protocols, ensuring a smooth and comfortable experience.
  3. Sample Tracking Updates
    You can track your sample and report status through the Metropolis Healthcare portal at every stage.
  4. Accurate Laboratory Testing
    Your sample is analysed using advanced PCR technology in NABL and CAP accredited laboratories for precise genetic detection.
  5. Quick & Easy Reports
    Reports are typically available within 24 hours via email, WhatsApp, or through the Metropolis Healthcare app and website.

Is Home Sample Collection for HLA B27 PCR Test Available Near You?

Metropolis Healthcare offers convenient home sample collection for the HLA B27 PCR Test. Our phlebotomists collect samples from your doorstep using sterile techniques, ensuring accuracy and patient comfort. This is especially helpful for patients with limited mobility or ongoing inflammation.

How Long Does It Take to Get an HLA B27 PCR Test Report?

Reports are usually available within 24 hours once the sample reaches the laboratory.
Note: Reporting time and cost may vary depending on your location.

Where Can I See or Get HLA B27 PCR Test Results?

You can easily access your test results through:

  • The Metropolis Healthcare website or app.
  • Email or WhatsApp once your report is ready.
  • Collecting a physical copy from your nearest Metropolis Lab.

Interpreting HLA B27 PCR Test Results

What Your HLA B27 PCR Test Results May Indicate

Result Interpretation
Positive The HLA-B27 gene is detected, indicating a genetic predisposition to autoimmune diseases such as Ankylosing Spondylitis or Reactive Arthritis.
Negative The HLA-B27 gene is not detected, suggesting no genetic association with these conditions.

 

Understanding Abnormal HLA B27 PCR Test Results by Parameter

 

Parameter Positive Result Negative Result
HLA-B27 Gene Indicates presence of genetic marker linked to autoimmune diseases Suggests no detectable genetic predisposition

 

How Should You Prepare for an HLA B27 PCR Test?

 

The HLA B27 PCR Test requires no special preparation. Follow these steps to ensure a smooth process:

  • No fasting required – you can eat and drink normally.
  • Inform your doctor about all current medications and supplements.
  • Stay relaxed and hydrated before your appointment.
  • Wear a short-sleeved shirt for easier blood draw access.

How Is an HLA B27 PCR Test Done? (Step-by-Step Procedure)

The HLA B27 PCR Test follows a simple, standard blood collection process:

  • Preparation: An elastic band is tied around your upper arm to make the vein visible.
  • Cleaning: The puncture site is cleaned with antiseptic to prevent infection.
  • Blood Draw: A sterile needle is inserted into your vein to collect blood.
  • Collection: About 3 ml of blood is drawn into an EDTA vacutainer.
  • Completion: The needle is removed, and a bandage is applied.
  • Processing: The sample is analysed using Real Time PCR (RT-PCR) to detect the HLA-B27 gene.

Conditions That May Affect HLA B27 PCR Test Accuracy

Although the HLA B27 PCR Test is highly accurate, a few factors may influence results:

  • Sample contamination during collection or handling.
  • Technical variations in PCR amplification.
  • Recent blood transfusion or bone marrow transplant.
  • Low DNA concentration or quality in the sample.

Diseases That an HLA B27 PCR Test Can Help Detect

The HLA B27 PCR Test helps doctors identify genetic susceptibility to several autoimmune and inflammatory diseases, including:

  • Ankylosing Spondylitis
  • Reactive Arthritis
  • Psoriatic Arthritis
  • Juvenile Idiopathic Arthritis
  • Uveitis (inflammatory eye disease)

Frequently asked questions

Find quick answers to the most common questions about health tests, bookings, and reports.

Regular exercise, a balanced diet, avoiding smoking, maintaining a healthy weight, and managing stress all help reduce heart disease risk.

Common signs include irritability, trouble sleeping, headaches, difficulty concentrating, and muscle tension.

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Ratings & Reviews

We're satisfying our customers every day since 1980.

Morning given the samples in Ayanavaram branch got the report by 12.30pm it's fast and efficient delivery by mail and want to tell about the staff they are very professional and friendly definitely I will recommend my friends and family to metropolis labs

Prabhu Gopalakrishnan. 5

The Home Service was excellent. Medical Technician took samples at 7:00am and returned at 9:30 after breakfast to take further samples. The results were sent by email at 6pm. The whole experience was excellent, professional and well organised. Will certainly recommend the home service for convenience

Chris Bessell 5

Excellent service - I was able to request services late at night (9pm) and the technician was at my door at 7:30am the next morning. The services were highly affordable and convenient. I live in the US and the US could learn a lot from Metropolis!!!

Sharmila Vaishnav. 5

Very good staff, excellent execution of samples, timely results even before promised time. professional and well organised. Will certainly recommend the home service for convenience

Ishwarakrishnan. 4

Very good staff, excellent execution of samples, timely results even before promised time. professional and well organised. Will certainly recommend the home service for convenience

Ishwarakrishnan. 4

Very good staff, excellent execution of samples, timely results even before promised time. professional and well organised. Will certainly recommend the home service for convenience

Ishwarakrishnan. 4

Choose precision On time, every time!

Precision
  • 98% on time collections with a 60 minutes promise
  • Real-time tracking of phlebotomist arrival and report generation
  • Fully digital report delivery—view from mobile or desktop instantly
  • Continuous monitoring of turnaround times to ensure accuracy and speed
  • Dedicated support team to resolve any delays or queries quickly

Why Metropolis Healthcare?

Metropolis has been present for over 40 years in the market.

Metropolis has a team of 200 senior pathologists and over 2000 technicians delivering diagnostic solutions in the areas of routine, semi specialty and super specialty domains like Oncology, Neurology, Gynaecology, Nephrology and many more.

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About Us

Metropolis Healthcare is a leading diagnostic service provider in India. We are a team of dedicated professionals committed to ensuring you and your family receive the most accurate and timely healthcare solutions. We believe in making health care accessible, affordable and evidence-based. Our expert panel are equipped with the latest technology and manned by a certified team of over 4000+ technicians.

In diagnostic services, we have more than three decades of experience servicing diverse stakeholders health advantage packages for men, women and seniors, genetic fitness & corporation in over 650+ tests like Oncology, Neurology, Gynaecology, Haematology, and many more. Our diagnostic services are located in leading doctors and clinics and serve over 15+ cities in India.

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