How is the blood draw procedure performed for the antinuclear antibody test?

A trained phlebotomist cleans your inner elbow, ties a band around your upper arm, and inserts a small needle to collect blood. The procedure takes only a few minutes with minimal discomfort.

Are there any risks to the test?

Risks are very low and similar to any standard blood test. You might experience minor pain, bruising, or slight bleeding at the needle site. Serious complications are extremely rare when proper procedures are followed.

What level of antinuclear antibody test is normal?

A negative result (index less than 1.0) is considered normal. Borderline results (1.0-1.2) and positive results (above 1.2) require a doctor's evaluation with clinical symptoms.

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Antinuclear Antibody (ANA) Test

₹ 1,050

Antinuclear Antibody (ANA) Test

₹ 1,050

51+ booked in last 3 days

Antinuclear Antibody (ANA) Test

Also Known As : ANA Test, ANA Screening, Antinuclear Antibody Screening

Report same day : 5:00pm

Fasting no

Sample Type serum

Recommended for Male, Female

ANA (Anti Nuclear Antibody) Screening by EIA, Serum

₹ 1,050

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Brief Details

Preparation

No special preparation required

Also Known As

ANA Test, ANA Screening, Antinuclear Antibody Screening

Fasting

Not required

Reporting Time

Same Day, 9:00 PM (if sample received before cutoff time)

Purpose

Helps diagnose autoimmune disorders, assists in evaluating systemic lupus erythematosus (SLE), and aids in assessing Sjögren's syndrome, rheumatoid arthritis, and other connective tissue diseases.

What Is an Antinuclear Antibody Test?

An antinuclear antibody (ANA) test is a blood test that detects specific proteins called autoantibodies in your blood. These antibodies mistakenly attack your body's own healthy cells instead of fighting infections. The test uses a serum sample drawn from a vein in your arm. This screening test helps doctors investigate and diagnose autoimmune diseases, particularly conditions like systemic lupus erythematosus (SLE), in which the immune system attacks the body's own tissues.

What Does the Antinuclear Antibody Test Measure?

The ANA test measures several important aspects of your immune system's activity:

Presence of autoantibodies: Proteins that target your own cell nuclei instead of foreign invaders.
Antibody concentration (titre): The level of these antibodies in your serum, expressed as an index value or dilution titre.
Antibody patterns: Characteristic staining patterns (e.g., homogeneous, speckled, nucleolar) that provide diagnostic clues for specific autoimmune diseases.
Index values: Numerical scores that help classify results as negative (<1.0), borderline (1.0–1.2), or positive (>1.2).

Antinuclear Antibody Test: Who Needs It and Why It's Done

What Symptoms May Call for an Antinuclear Antibody Test?

Your doctor may recommend an ANA test if you experience persistent symptoms suggestive of autoimmune or connective tissue disorders:

Unexplained fever that doesn't go away
Extreme fatigue that rest doesn't improve
Joint pain, stiffness, and swelling similar to arthritis
Butterfly-shaped rash across your cheeks and nose
Other skin rashes or sensitivity to sunlight
Muscle pain and weakness
Unexplained hair loss
Fingers and toes turning white or blue in cold weather (Raynaud’s phenomenon).

Who Should Get an Antinuclear Antibody Test?

The ANA test is recommended for specific groups of people:

Patients with symptoms: Individuals experiencing multiple unexplained symptoms that could indicate autoimmune disease.
Family history: People with close relatives who have been diagnosed with autoimmune conditions.
High-risk individuals: Those with other autoimmune markers or related health concerns.
Follow-up testing: Patients monitoring existing autoimmune conditions.

Why Is an Antinuclear Antibody Test Done?

The ANA test serves as a preliminary screening tool to detect autoimmune activity; it cannot confirm a diagnosis on its own. It guides further testing and helps establish an accurate diagnosis for proper treatment.

Importance of an Antinuclear Antibody Test

The ANA test is clinically significant as one of the most sensitive screening tools for autoimmune diseases such as systemic lupus erythematosus (SLE). While a positive result doesn't diagnose a specific disease, it serves as an important signpost for doctors. It helps narrow down possible causes of your symptoms and justifies ordering more specific autoantibody tests.

Early and accurate diagnosis through ANA testing is essential for managing autoimmune diseases effectively and preventing long-term organ damage.

Antinuclear Antibody Test Booking & Reports – Metropolis Healthcare India

How to Book an Antinuclear Antibody Test?

Simple Online Booking
You can book the test through the Metropolis Healthcare App or website. Select 'Antinuclear Antibody Test', choose a convenient time slot, and provide your address for a blood test at home. You can also visit the nearest Metropolis Lab if you prefer to give the sample directly at the lab.
Safe Home Sample Collection
Our trained phlebotomists ensure the timely collection of your sample while strictly following all safety and hygiene protocols.
Sample Tracking Updates
Stay informed at every step — from collection to testing — by tracking your sample directly through the Metropolis Healthcare website.
Accurate Laboratory Testing
Your sample is processed at our NABL and CAP-accredited laboratories, where expert technicians ensure accurate results.
Quick & Easy Reports
Receive your test reports promptly via email, WhatsApp, or by downloading them directly from the Metropolis Healthcare website or app.

Is Home Sample Collection for the Antinuclear Antibody Test Available Near You?

Yes, home sample collection is available for the ANA test across India through Metropolis Healthcare. Our trained phlebotomists follow strict safety protocols while collecting your blood sample and processing it in NABL- & CAP-accredited labs. With our extensive network of over 10,000 touchpoints in 750 towns, our reliable home collection services ensure you receive accurate results without compromising on quality or convenience.

How Long Does It Take to Get an Antinuclear Antibody Test Report?

Reports are typically available the same day by 9:00 PM once the sample reaches the lab.

Where Can I See or Get the Antinuclear Antibody Test Results?

Test results from Metropolis Healthcare can be accessed through multiple convenient channels. You can log in to the Metropolis website using your credentials or use the Metropolis Healthcare App to view and download your reports. Additionally, test reports are sent via email or WhatsApp, and you also have the option to collect a physical copy directly from the lab.

Interpreting Antinuclear Antibody Test Results

The following table explains what your ANA test results mean and how they are interpreted:

Parameter	Reference Range	Low Values	High Values
Antinuclear Antibody (Index)	Negative: < 1.0 Borderline: 1.0-1.2 Positive: > 1.2	Low values are often seen in healthy individuals without autoimmune disease and are unlikely to be clinically meaningful.	An index > 1.2 suggests the presence of autoantibodies, requiring further evaluation for autoimmune conditions.

Conditions that May Affect Antinuclear Antibody Test Accuracy

Several factors can influence your ANA test results and should be discussed with your doctor:

Certain medications, including some blood pressure drugs, anti-seizure medicines, and antibiotics, can cause drug-induced lupus.
Age-related factors, such as low-level ANA, can be found in some healthy elderly individuals.
Recent infections or illnesses may temporarily affect antibody levels.
Pregnancy can sometimes influence autoantibody production.
Severe dehydration may affect blood concentration, though it is unlikely to change qualitative results.
Some herbal supplements and over-the-counter medications may interfere with results.

How Is an Antinuclear Antibody Test Done?

The ANA test involves a simple blood collection procedure performed by trained professionals:

A phlebotomist cleans the area on your inner elbow with an antiseptic.
An elastic band is tied around your upper arm to make the veins more visible.
A small needle is inserted into a vein to collect approximately 3 ml of blood.
The blood sample is collected in a gel vacutainer to separate serum for analysis.
Your serum sample is analyzed using the Enzyme Immunoassay (EIA) method (ID-based qualitative screening test).
If the method is not specified, preference is given to the Indirect Immunofluorescence (IF) technique.

How Should You Prepare for an Antinuclear Antibody Test?

Preparing for your ANA test is straightforward with minimal requirements:

No fasting is required; you can eat and drink normally before the test.
Continue regular medications unless your doctor specifically advises otherwise.
Inform your doctor about all medications, supplements, and herbal remedies you're taking.
Stay well-hydrated before the test, as good hydration makes venipuncture easier.
Wear comfortable clothing with sleeves that can be easily rolled up.
Bring your prescription and any relevant medical history to your appointment.

Diseases that an Antinuclear Antibody Test Can Help Detect

The ANA test serves as a screening tool for various autoimmune and connective tissue disorders:

Systemic Lupus Erythematosus (SLE)
Sjögren's syndrome
Rheumatoid arthritis
Systemic sclerosis (scleroderma)
Mixed connective tissue disease
Autoimmune hepatitis (AIH)
Primary biliary cholangitis
Drug-induced lupus
ANA-associated vasculitis

Antinuclear Antibody Tests/Packages that You Can Book With Metropolis Healthcare

Here are the ANA-related tests and packages available with Metropolis Healthcare:

Test/Package Name	Purpose/Highlights
ANA (Antinuclear Antibody) Test	Basic screening for autoimmune disorders
ANA Blot 18 Antigen Test	Comprehensive panel identifying 18 specific autoantibodies.
ANA Profile Test	Detailed evaluation of specific autoantibodies such as anti-dsDNA, anti-Sm, anti-Ro/SSA, and anti-La/SSB.

Frequently asked questions

Find quick answers to the most common questions about health tests, bookings, and reports.

There is no specific best time for an ANA test. It can be performed at any time of day. Follow your doctor’s advice or the diagnostic lab’s scheduling guidelines

No, fasting is not required for a standalone ANA test. You can eat and drink normally before the test. However, if other tests requiring fasting are ordered simultaneously, follow those specific instructions.

While severe dehydration can affect blood concentration, it's unlikely to change a qualitative result from negative to positive. However, staying well-hydrated before any blood test ensures accuracy and makes blood collection easier.

No dietary changes are needed. However, inform your doctor about all medications, including prescription drugs, over-the-counter medicines, and herbal supplements, as some can cause false-positive results. Don't stop medications unless advised.

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AMH (Mullerian Inhibiting Substance), Serum Amylase, Serum ANA (Anti Nuclear Antibody) Screening by EIA, Serum Calcium, Serum CCP Antibody, Serum Cholesterol Total, Serum C-Peptide - Fasting, Serum Creatinine, Serum CRP (C Reactive Protein), Serum D Dimer - Quantitative, Citrated Plasma Dengue NS1 Antigen, Serum ESR (Erythrocyte Sedimentation Rate), EDTA Blood Ferritin, Serum Glucose Fasting, Fluoride Plasma Glucose Random, Fluoride Plasma HCV (Hepatitis C Virus) - Total antibody, Serum HIV-DUO (IV th Generation test), Serum Homocysteine, Serum Mantoux Tuberculin Skin Test (2TU) NT-Pro BNP, Serum PAP smear by Conventional method Prolactin, Serum Total PSA (Prostate Specific Antigen), Serum SGOT (AST), Serum SGPT (ALT), Serum Total Testosterone by ECLIA, Serum Troponin-I, Serum TSH (Thyroid Stimulating Hormone) - Ultrasensitive, Serum Typhi Dot - IgM antibody, Serum Uric Acid, Serum VDRL (RPR), Serum Vitamin B12 (Cyanocobalamin), Serum Vitamin D Total - 25 Hydroxy (OH) , Serum aPTT (Activated Partial Thromboplastin Time), Citrated plasma Bilirubin Total, Direct, Indirect, Serum CBC Haemogram, EDTA Blood Culture & Sensitivity - Aerobic bacteria, Urine Prega screen (Dual Marker) Reflex - NIPT or Karyotyping Pregascreen (Quadruple Marker), Serum Electrolytes, Serum Food Intolerance (Food IgG) Test, Serum HbA1c- Glycated Haemoglobin, EDTA Blood HCG Beta Subunit, Serum Hemoglobin, EDTA Blood HOMA Index (Insulin Resistance Test), Flouride Plasma Iron Studies, Serum Lipid Profile - 2 (Mini : Fasting), Serum Malaria Parasite Detection by Smear Examination PT (Prothrombin Time), Citrated Plasma SARS- CoV-2 (COVID 19) RNA Detection by RT-PCR, VTM Swab Thyroid Profile - 1 Urea, Serum Widal Test for Typhoid, Serum

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Test - Clonidine Growth Hormone Stimulation Test - Exercise Growth Hormone Stimulation Test - GHRH Growth Hormone Stimulation Test - Insulin Growth Hormone Stimulation Test - L Dopa Growth Hormone Suppression Test - Glucose GTT (Glucose Tolerance Test) Profile - 2 Hrs (75 grams glucose) GTT (Glucose Tolerance Test) Profile - 3 Hrs (100 grams glucose) GTT (Glucose Tolerance Test) Profile - 2 Hrs, 2 sample Brain Tumor Panel - 2 Brain Tumor Panel - 1 Haemophilia Profile HCG Stimulation Test Profile for DHT HCG Stimulation Test - FSH and LH HCG Stimulation Test - Testosterone HCV (Hepatitis C Virus) Genofibro Profile HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HCV (Hepatitis C Virus) Combo Profile - 1 HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Immunity Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Chronic Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV (Hepatitis B Virus) Acute Profile HBV 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B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Homocysteine reflex B12-folate Profile Lung Carcinoma (NSCLC) Panel Head and neck cancer (HNSCC profile) H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, HBV, HCV) Viral marker Profile H3 (HIV, 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Antinuclear Antibody (ANA) Test

Brief Details

What Is an Antinuclear Antibody Test?

What Does the Antinuclear Antibody Test Measure?

Antinuclear Antibody Test: Who Needs It and Why It's Done

What Symptoms May Call for an Antinuclear Antibody Test?

Who Should Get an Antinuclear Antibody Test?

Why Is an Antinuclear Antibody Test Done?

Importance of an Antinuclear Antibody Test

Antinuclear Antibody Test Booking & Reports – Metropolis Healthcare India

How to Book an Antinuclear Antibody Test?

Is Home Sample Collection for the Antinuclear Antibody Test Available Near You?

How Long Does It Take to Get an Antinuclear Antibody Test Report?

Where Can I See or Get the Antinuclear Antibody Test Results?

Interpreting Antinuclear Antibody Test Results

Conditions that May Affect Antinuclear Antibody Test Accuracy

How Is an Antinuclear Antibody Test Done?

How Should You Prepare for an Antinuclear Antibody Test?

Diseases that an Antinuclear Antibody Test Can Help Detect

Antinuclear Antibody Tests/Packages that You Can Book With Metropolis Healthcare