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Vitamin D Plus Profile Test
₹ 1,920
Vitamin D Plus Profile Test
₹ 1,920
51+ booked in last 3 days

Vitamin D Plus Profile Test

Also Known As : Vitamin D Profile Test, Vitamin D and PTH Test, Vitamin D and Calcium Metabolism Panel

Report
Report after 30 hours
Fasting
Fasting no
Recommended for
Recommended for Male, Female
Vitamin D Plus Profile
₹ 1,920

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Brief Details

Preparation
Avoid high-dose biotin supplements (>5 mg/day) at least 8 hours before the test
Also Known As
Vitamin D Profile Test, Vitamin D and PTH Test, Vitamin D and Calcium Metabolism Panel
Fasting
Fasting not required
Reporting Time
After 6 hours
Purpose
To assess Vitamin D levels and parathyroid hormone balance for evaluating bone health and calcium metabolism

What is a Vitamin D Plus Profile Test?

The Vitamin D Plus Profile Test is a blood test that checks your body’s Vitamin D and Parathyroid Hormone (PTH) levels to provide a combined view of bone health and calcium metabolism. Vitamin D supports calcium absorption and bone mineralisation, while PTH regulates calcium and phosphate balance in the bloodstream.

Testing both markers together helps your clinician interpret bone pain, muscle weakness, suspected deficiency, or abnormal calcium results more accurately than Vitamin D testing alone. The test requires a blood sample drawn from a vein in your arm.

What Does a Vitamin D Plus Profile Test Measure?

This profile measures two key markers involved in calcium and bone metabolism:

  • 25 OH Vitamin D: The major circulating form of Vitamin D and the best indicator of overall Vitamin D stores.
  • Parathyroid Hormone (PTH): A hormone that regulates calcium and phosphate; helps interpret whether the body is compensating for low Vitamin D or calcium imbalance.
  • Combined Assessment: Helps distinguish Vitamin D deficiency from parathyroid-related disorders and other metabolic causes of bone symptoms.

Vitamin D Plus Profile Test: Who Needs It and Why It’s Done

What Symptoms May Call for a Vitamin D Plus Profile Test?

Your doctor may recommend this test if you experience:

  • Bone or joint pain
  • Muscle weakness or cramps
  • Chronic fatigue or low energy
  • Frequent fractures or poor bone healing
  • Soft bones (osteomalacia) or weak teeth
  • Signs or risk of osteoporosis

Who Should Get a Vitamin D Plus Profile Test?

This test is especially relevant for:

  • Individuals with low sunlight exposure
  • Older adults and postmenopausal women
  • People with kidney or liver disorders
  • Patients with suspected calcium or Vitamin D imbalance
  • Individuals taking long-term steroids or anti-seizure medicines
  • People with known/suspected parathyroid or thyroid disorders

Why is a Vitamin D Plus Profile Test Done?

It evaluates Vitamin D–PTH interaction to detect deficiency or excess, diagnose bone/mineral metabolism disorders, and guide supplementation and treatment decisions.

Importance of a Vitamin D Plus Profile Test

Measuring Vitamin D with PTH provides stronger clinical context than Vitamin D alone. For example, low Vitamin D with high PTH commonly suggests compensatory (secondary) hyperparathyroidism, while abnormal PTH with calcium abnormalities can point toward parathyroid gland disorders. This supports more precise diagnosis and monitoring of therapy response.

Vitamin D Plus Profile Test Booking & Reports – Metropolis Healthcare

How to Book the Vitamin D Plus Profile Test and Get Your Reports?

  1. Simple Online Booking
    Book via the Metropolis Healthcare website or app. Select the Vitamin D Plus Profile Test, choose a slot, and opt for home collection or visit a nearby lab.
  2. Safe Home Sample Collection
    Trained phlebotomists collect the blood sample using sterile technique and standard biosafety precautions.
  3. Sample Tracking Updates
    Track sample status through the Metropolis platform (website/app).
  4. Accurate Laboratory Testing
    Testing is performed at NABL & CAP accredited laboratories using immunoassay methods (e.g., ECLIA) for measurement.
  5. Quick Report Access
    Reports are typically delivered via email/WhatsApp and available for download in the Metropolis portal/app.

Is Home Sample Collection for Vitamin D Plus Profile Test Available Near You?

Yes. Home blood collection is availabe for Vitamin D Plus Profile.

How Long Does It Take to Get a Vitamin D Plus Profile Test Report?

Reports are usually available within 6 hours once the sample reaches the lab.
Note: Reporting time and cost may vary based on your location.

Where Can I See or Get Vitamin D Plus Profile Test Results?

Results can be viewed/downloaded through the Metropolis website/app and are often shared via email or WhatsApp. Printed copies may be collected from the lab if required.

Interpreting Vitamin D Plus Profile Test Results

What Your Vitamin D Plus Profile Test Results May Indicate

Parameter

Normal Range

Low Values May Indicate

High Values May Indicate

25 OH Vitamin D

30–100 ng/mL

Vitamin D deficiency, poor calcium absorption, weak bones

Vitamin D toxicity or excessive supplementation

PTH

15–65 pg/mL

Hypoparathyroidism, high calcium levels

Secondary hyperparathyroidism due to Vitamin D deficiency or chronic kidney disease

 

Understanding Abnormal Vitamin D Plus Profile Test Results by Parameter

Parameter

Low Values

High Values

Possible Interpretation

25 OH Vitamin D

Deficiency (<10 ng/mL) or insufficiency (10–30 ng/mL)

Hypervitaminosis D (>100 ng/mL)

Low levels may reflect poor sunlight exposure, malabsorption, or dietary deficiency; very high levels can reflect over-supplementation.

PTH

<15 pg/mL

>65 pg/mL

Low PTH can suggest hypoparathyroidism (or other metabolic issues such as magnesium deficiency); high PTH often indicates secondary hyperparathyroidism due to Vitamin D deficiency or CKD.

 

How Should You Prepare for a Vitamin D Plus Profile Test?

  • No fasting required; you can eat and drink normally.
  • Avoid high-dose biotin (>5 mg/day) for at least 8 hours before the test (biotin can interfere with some immunoassays).
  • Inform your clinician about Vitamin D, calcium, and other supplements you are taking.
  • Maintain normal hydration to support an easier blood draw.
  • Avoid recent high-dose Vitamin D injections unless prescribed; disclose any recent dosing to your clinician.

How Is a Vitamin D Plus Profile Test Done? (Step-by-Step Procedure)

  1. Preparation: A tourniquet is applied to make veins more prominent.
  2. Skin cleansing: The site is cleaned with antiseptic.
  3. Sample collection: Blood is drawn (commonly ~3 ml) into a gel vacutainer.
  4. Processing: Serum is analysed using immunoassay technology (e.g., ECLIA) to measure 25 OH Vitamin D and PTH.
  5. Reporting: Results are issued with reference ranges for clinician interpretation.

Conditions That May Affect Vitamin D Plus Profile Test Accuracy

  • High-dose biotin intake within 8 hours prior to testing
  • Severe acute illness, prolonged stress, or poor nutritional status
  • Medications affecting Vitamin D/PTH (e.g., steroids, anti-seizure drugs, some diuretics)
  • Chronic kidney disease (can alter PTH and Vitamin D metabolism and interpretation)

Diseases That a Vitamin D Plus Profile Test Can Help Detect

  • Vitamin D deficiency or insufficiency
  • Osteoporosis and osteomalacia (soft bones)
  • Rickets (in children)
  • Secondary hyperparathyroidism
  • Hypoparathyroidism
  • Chronic kidney disease–mineral and bone disorder (CKD-MBD)
  • Calcium metabolism disorders (as part of broader evaluation)

Vitamin D Plus Profile Test Price in Different Cities

The cost of a Vitamin D Plus Profile Test may vary depending on your location. Here's a table showing the approximate price range for the Vitamin D Plus Profile Test at Metropolis Healthcare in major Indian cities:

City

Approximate Cost (INR)

Mumbai

₹ 1,600 to ₹ 2,200

Delhi

₹ 1,600 to ₹ 2,200

Bengaluru

₹ 1,600 to ₹ 2,200

Chennai

₹ 1,600 to ₹ 2,200

The Vitamin D Plus Profile Test cost may vary depending on the city and the specific tests included in the panel. However, Metropolis Healthcare offers competitive Vitamin D Plus Profile Test prices across major cities in India.

References

  1. Holick M.F., et al. (2011). Evaluation, Treatment, and Prevention of Vitamin D Deficiency: Endocrine Society Clinical Practice Guidelines. Journal of Clinical Endocrinology & Metabolism, 96(7), 1911–1930. PMID: 21646368
  2. Institute of Medicine (US). (2011). Dietary Reference Intakes for Calcium and Vitamin D. National Academies Press. PMID: 21796828
  3. Lips P. (2001). Vitamin D deficiency and secondary hyperparathyroidism in the elderly: Consequences for bone loss and fractures. Clinical Endocrinology, 54(4), 401–407. PMID: 11318809
  4. Souberbielle J.C., et al. (2010). Vitamin D and PTH relationship: The threshold for PTH suppression. PLOS ONE, 5(6), e11012. PMID: 20559536
  5. Bilezikian J.P., et al. (2018). Management of Hypoparathyroidism. Endocrine Practice, 24(5), 529–539. PMID: 29714536

Frequently asked questions

Find quick answers to the most common questions about health tests, bookings, and reports.

It measures 25 OH Vitamin D and Parathyroid Hormone (PTH) to assess Vitamin D status and calcium-bone metabolism together.

No. Fasting is not required.

PTH reflects how the body is regulating calcium and phosphate. When interpreted with Vitamin D, it helps identify whether symptoms are due to deficiency, parathyroid dysfunction, or other metabolic causes.

This commonly suggests Vitamin D deficiency with secondary hyperparathyroidism (a compensatory rise in PTH to maintain calcium balance).

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Metropolis has been present for over 43 years in the market.

Metropolis has a team of 200 senior pathologists and over 2000 technicians delivering diagnostic solutions in the areas of routine, semi specialty and super specialty domains like Oncology, Neurology, Gynaecology, Nephrology and many more.

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